Simultaneous Analysis of Multiple Endogenous Plant Hormones in Leaf Tissue of Oilseed Rape by Solid

发布时间：2018/1/16 点击次数：532

Introduction – Plant hormones are important signalling molecules that act at lower concentrations to regulate numerous plant physiological and developmental processes. In order to study the functions of plant hormones, it is necessary to develop a high‐throughput and highly selective and sensitive method for determination of plant hormones. Objective – Based on SPE‐HPLC‐ESI‐MS/MS method, a highly selective and sensitive method for determination of six plant hormones in leaf tissue of oilseed rape was developed. Methodology – The extraction was performed with C18 solid‐phase extraction cartridge and the sample was subsequently analysed by HPLC‐ESI‐MS/MS. Methanol and H2O with 0.05% formic acid was selected as the phase for HPLC, using a gradient of increasing methanol content. The plant hormones were quantified in MRM mode and identified in IDA mode by the hybrid triple quadrupole/linear ion trap mass spectrometer with high sensitivity and selectivity. Results – Under the optimal conditions, good linearities were obtained for six plant hormones with the correlation coefficients above 0.9924. The detection limits of the target compounds were in the range of 0.005–0.2 ng/mL. Reproducibility of the method was obtained with intra‐day and inter‐day relative standard deviations. The extraction recovery yields of plant hormones under SPE conditions ranged from 67.03 to 119.83%. Compared with previous methods, sample preparation time and amount of sample required for analysis of plant hormones were reduced, and more classes of hormones were quantitatively measured.（前言：植物激素是重要的信号分子,它们在低浓度的作用下调节大量的植物生理和发育过程.为了研究植物激素的作用,开发一种高通量、高选择性和灵敏的植物激素测定方法是有必要的。目的：根据SPE‐HPLC‐ESI‐MS/MS 方法,开发出一种高选择性和敏感的方法,用于测定油籽油菜叶组织中6种植物激素的含量。方法：采用C18固相萃取法进行萃取,然后采用HPLC-ESI-MS/MS对样品进行分析。HPLC的流动相为：甲醇,水和0.05%的甲酸,采用增加甲醇量的梯度；植物激素在MRM模式下被量化,在IDA模式下被鉴别。）

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