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The impact of long term exposure to phthalic acid esters on reproduction in Chinese rare minnow (Go
发布时间：2018/1/16 点击次数：466
The environmental risk of phthalic acid esters (PAEs) is of great concern. We investigated the reproductive impairment of di-(2-ethylhexyl)-phthalate (DEHP) on Chinese rare minnow, an endemic fish inhabiting the upper streams of the Yangtze River. Chinese rare minnow larvae were exposed to environmentally relevant concentrations of DEHP (0, 4.2, 13.3, and 40.8 mg/L) for 6 months. Plasma testosterone and 17b-estradiol levels decreased in females, accompanied by downregulation of cyp19a and cyp17 gene transcription in ovary. Increases in plasma testosterone concentration were observed in males, accompanied by downregulation of cyp19a gene transcription in testes. Hepatic VTG gene transcription was upregulated in males and females. Exposure to DEHP reduced egg production and inhibited oocyte maturation in females and retarded spermiation in males. Decreased egg protein content was measured in F1 embryos. These results indicate that long-term exposure to low concentrations of DEHP (13.3 mg/L) causes endocrine disruption and impairs fish reproduction.（邻苯二甲酸酯引起的环境风险是非常值得关注的。 我们研究了邻苯二甲酸二酯对中国稀有鱼类鲦鱼造成的生殖损伤,这种鱼生活在长江的上游。中国稀有鲦鱼小鱼苗在邻苯二甲酸二酯浓度分别为(0, 4.2, 13.3, and 40.8 mg/L)的环境下六个月。雌鱼的血浆睾酮和17β雌二醇水平降低,连同卵巢上的cyp19a 和cyp17基因转录也有所下降。雄鱼中血浆睾酮浓度增加,连同睾丸上的cyp19a基因转录也有所下降。雌雄鱼中的肝VTG基因转录上调。在邻苯二甲酸二酯环境中,减少了卵子的产生,抑制了雌性卵细胞的成熟,延缓了雄性精子的发育。在F1胚胎中蛋白含量减少。这些结果显示长期接触低浓度邻苯二甲酸二酯(13.3 mg/L)能引起鱼类的内分泌失调和损害鱼的繁殖。）
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