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LIPASE CATALYZED SYNTHESIS OF ABA-TYPE STRUCTURED LIPID FROM SINGLE CELL OIL AND TRIPALMITIN(脂肪酶催化合成
发布时间：2018/3/1 点击次数：562
Optimization of lipase-catalyzed interesterification for the production of structured lipid, enriched with arachidonic acid (ARA) from tripalmitin and ARA single cell oil was carried out. Response surface methodology was applied to model and optimize the reaction system; factors of temperature, substrate molar ratio, reaction time and enzyme load were chosen for the optimization. The optimal conditions generated from the models were as follows: temperature of 50C, substrate molar ratio of 1 : 12, reaction time of 13.36 h, and enzyme load of 7.58 wt %. Under these conditions, the incorporation of ARA and acyl migration were 55.1160.15 and 15.8560.05%, respectively. It was found that 69.57% of ARA was mainly incorporated in the sn-1, 3 positions and 62.31% of palmtic acid was located at the sn-2 position of the TAG.(应用响应面分析法被应用于模型和优化反应系统：温度,底物摩尔比,反应时间,酶量反应条件的优化。模型生成的*条件如下:50℃,摩尔比1:12,反应时间13.36h,酶量是7.58wt%。在这些条件下,ARA和酰基位移的结合分别是55.11±0.15 and 15.85±0.05%。69.57%ARA主要是合并在sn-1, 3位置,62.31%十六酸在sn-2位置。)
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